Set up the octet using the lcd screen and menu options. The fmg interaction with pten protein was analyzed by octet red fortebio a the association and dissociation curves and b affinity of the fmg and pten protein were calculated via the fortebio software. The xx here represents the last octet of the mac address. The octet may also be setup and controlled serially. Octet red384 getting started introduction load analyte sample. See ethernet ip publications and this user manual for further guidance. Small molecule kinetics can be measured on the octet red, octet red96 and octet red384 instruments. Used analyzers the lab world group used lab equipment. Rt100v8 petscan rt100v8 user manual realtrace realtrace petscan rt100v8. Get accurate concentration, kinetic constants, and affinity data for proteinprotein, small moleculeprotein and other fastbinding interactions all with dip and read simplicity. Pall fortebios octet red384 and octet qk384 systems are designed for increased throughput for labelfree protein quantitation and kinetic characterization. The dos ping command can be used to check if an ip address is currently used or vacant see page 4. In the main menu select help data acquisition user. An ipv4 address comprises 4 8bit numbers separated by a period.
View and download promise technology smartstor ns4600 product manual online. It is ideal for rapidly measuring concentration of proteins and other biomolecules, measuring kinetics and affinity, and screening proteinprotein and proteinsmall molecule interactions. Refer all servicing to qualified service personnel. Our products enable scientists to improve productivity and efficiency in scientific discovery and research. The rdb4078 is designed to interface seamlessly with the red suite 5 and lpcxpresso 5 ides from code red technologies. Ensure each receiver has a unique address see receiver manual or appendix b. The octet red96e system is designed to work reliably in regulated environments. Each number can be between 0 and 255 some of the fields have more strict stride industrial ethernet switches user manual 2nd ed. The component formula of salvia miltiorrhiza and panax. This kit is used for testing for free available chlorine, monochloramine, dichloramine, total residual chlorine, and ph using dpd tablets, phenol red tables and the octaslide 2 comparator. The latest release, version 11, is available for use on all octet systems. Black box servswitch octet kv1700 user manual pdf download. The octet red384 is a nonfluorescencebased, highthroughput, bench top, protein interaction detection system that uses biolayer interferometry bli technology, and can analyze up to 16 samples in parallel for affinity, kinetics or active protein concentration determination from samples in 96 or 384well microplates. Includes 1 each of 181127 performance qualification kit quantitation and 181128 performance qualification kit kinetics with all necessary reagents, biosensors, microplates, manuals and cdrom for performing two instrument qualifications.
Table of recommened sensor offsets by well volume from octet user manual. Nxiq nx5664 module with framerelay performance monitoring 1200. Servswitch octet kv1701, servswitch octet kv1702, servswitch octet kv1703, servswitch octet kv1712, servswitch octet kv17, servswitch octet. When using the static rotary mode, the last octet of the modules ip address can be set via the rotary codingswitches on the module.
Confidential information the octet software kinetics analysis and reporting user software enabling graph exporting in a number of different formats streamlined data analysis and model fitting within origin 7. Who should use this manual this manual is intended for qualified personnel. Each instrument comes fully tested and backed by warranty. Octet red384 getting started introduction the octet red384 is an instrument for biolayer interferometry bli, an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. Tberd 307 ds3ds1 analyzer users manual august 1997 software level b. The red bars at the top and bottom of a column indicate the.
This user manual describes how to install, configure, and troubleshoot your 48cr code reader device. An astriban k is connected to the asteri sk server via a usb 2. Led blue bluetooth activated led red charging battery led green battery charged mini usb interface scroll up button 2 mid button 4 enter scroll down button 3 iso fdxb tag 260220 reader characteristics frequency 4. In a typical experiment, a biotinylated protein target is immobilized onto a highcapacity super streptavidin ssa biosensor surface, and this surface is exposed to a solution of the small molecule in a microplate well. The forte octet red 96 is capable of analyzing multiple samples in a 96well format.
Molecular devices is one of the leading provider of highperformance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. Captureselect fcxl affinity matrix thermo fisher scientific. Octet binding curve rate of increase in optical thickness as the. In the periodic table, the number of valence electrons is given by the group number. Servicing is required when the apparatus has been damaged in any way, such as power supply cord or plug is damaged, liquid has been spilled or objects have fallen into the apparatus, the apparatus has been exposed to rain or moisture. Cip safety encoder user manual rockwell automation. Biolayer interferometry bli using the octet red96e system.
Simply connecting a pc to the rdb4078 to the usb debug port enables the code red ide v5 to be used as your code development and debugging environment. Measured using the octet red system, the affinity constant kd of fcgr3a bound to human igg1 was 180nm. It only shows the setting fields that are required for creating the group. Select this option to save data in the earlier octet red soft ware 5. Fortebio octet data acquisition and data analysis software is rapidly evolving to better address the growing applications and needs of our customers. View and download black box servswitch octet kv1700 user manual online. The octet red96e system is microfluidicsfree, thereby reducing assay complexity, eliminating timeconsuming cleaning steps and cutting operating costs. The octet red384 is an instrument for biolayer interferometry bli, an optical. Promise technology smartstor ns4600 product manual. The add group dialog appears when adding a new group to a parent group. Click the settings tab of a group to change settings add group. Twokit bundle for kinetics and quantitation performance qualification for octet red96e system. Covalent bonding, octet rule, polarity, and basic types of formulas valence electrons they are those found in the highest energy level of the atom, or outer shell.
For updates about all uwmadison campus operations related to covid19, see covid19. Set up your printer and install the printer drivers onto your pc as described in the printers setup guide or user manual. These comparison tables provide a general guide to the features included in various versions of the software. Biomolecular binding kinetics assays on the octet platform 2 of lead candidates during development. All other trademarks belong to their respective owners. Interferometry, biolayer biophysics instrumentation. I have a airlap1242agek9 that i was working to flash to the autonomas version ap1242ag and during that process i guess i held the mode button down longer than 2 seconds when i tried to factory reset it as now everytime i boot it and connect the ethernet cable the led starts fla.
The octet data analysis ht software is designed for fast analysis of large datasets from quantitation, kinetic and epitope binning assays. A solid red light indicates that the device was not able to achieve a normal operating state. Install receiver units per the instructions in the respective receiver user manual. Accordingly, downstream processes require detailed characterization of specificity, selectivity, stability and binding rate analyses under various growth or buffer conditions to facilitate informed process decisions. General requirement seeking contractor to provide all labor, tools, material, parts, equipment, supervision, software, license, certification, transportation, management, and personnel required for a preventive maintenance on government equipment octet red384 system, located in bldg. The lab world group sells a variety of used analyzers including agilent bioanalyzer, food safety analyzers, hematology and chemistry analyzers. General basic information about the method of operation, fitting, installing and commissioning cpx terminals can be found in the cpx system manual. Servswitch octet kv1700 switch pdf manual download. The ip address for each lin501c module in an installation must be unique and not conflict with other devices on the network. Addresses 0 and 255 are reserved and cannot be used. Fortebios octet red96 system is a multifunctional, labelfree, realtime analysis instrument.
Power up the device, and wait for the module status indicator to blink red, the network status indicator to turn off, and the link status indicators to. Biolayer interferometry bli is used to measure affinities, on and off rates and quantitation. In response to the covid19 situation, uwmadison is suspending facetoface instruction beginning march 23. System requirements the router operates with most computers and wireless devices.
Dissolve the protein in sterile double distilled water to a concentration of 0. Simple western analyses were performed according to the proteinsimple user manual 44, 45. It supports analog telephone sets, analog lines a pstn connection, isdn pri and bri lines, e1 r2 lines, and isdn bri telephone sets. The get red y software for parameterizing and operating the smartdevices driver for the usb communication cable, type pdmu operating instructions. Unplug this apparatus during lightning storms or when unused for long periods of time. Plate for buffers, reagents and samples standard 96 well plate, greiner catalog number 655209, for redred96red96e. Fcgr3a cd16a recombinant human protein, 176 val, his tag. The 48cr code reader can only be used in ethernetip systems. In addition, the sample material remains available after analysis for further investigation. Airlap1242agek9 led flashing amberred i need to reload. Octet red96e performance qualification set molecular. Servswitch octet kv1701, servswitch octet kv1702, servswitch octet kv1703, servswitch octet kv1712, servswitch octet kv17, servswitch octet kv1711. North america, conference, boston, ma may 04 may 08, 2020 more than 2,800 worldrenowned experts, visionaries, and influencers from top pharma, biotech, academic, and government institutions convene in boston each spring for pegs.
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